NUI
No nā nīnau e pili ana i kā mākou huahana a i ʻole ka papa inoa kumukūʻai, e ʻoluʻolu e waiho i kāu leka uila iā mākou a e hoʻopili mākou i loko o 24 mau hola.
NINAU ANOKūlana halihali
Hiki i ka wela maʻamau
Manaʻo i ke ʻano hoʻouna
Ma ka lewa Ma ka aina Ma ke kai
Kūlana mālama:
E mālama i kahi pōʻeleʻele, Inert ea, lumi wela
Ola ola
Ma kahi o 2 makahiki
Ka nui o ke kauoha liʻiliʻi:
1kg (kūkākūkā)
palapala hōʻoia:COA, wahi hehee, Hoʻololi kikoʻī [α]D20
E hoʻouna mākou i nā ʻikepili āu e makemake ai.Hiki iā ʻoe ke ʻike i ka ʻikepili ma mua ma ke ʻano he kuhikuhi.
No nā huahana maʻamau, hoʻouna mākou i ka ʻikepili hou ma hope o ka puka ʻana mai.
FMOC-OT-BUTYL-D-TYROSINE;
FMOC-O-TERT-BUTYL-D-TYROSINE;
FMOC-D-TYR(TBU)-OH99%;
N-α-Fmoc-O-tert-butyl-D-tyrosine;
NALPHA-9-Fluorenylmethoxycarbonyl-O-tert-butyl-D-tyrosine;
9-fluorenylmethyloxycarbonyl-D-tyrosine(tert-butyl)-OH;
(R)-2-(((9H-fluoren-9-yl)methoxy)carbonylamino)-3-(4-tert-butoxyphenyl)propanoicacid;
Fmoc-D-Tyr(tBu)-OH(Fmoc-O-tert-butyl-D-tyrosine)
Hoʻohana pinepine ʻia lākou no ka hoʻopili ʻana i ka pauka.Pili ia i nā koi o nā mea kūʻai aku.No ka mālama pono i ka hōʻea ʻana i kahi e hele ai.E hoʻopaʻa mākou i nā ʻōmole plastik me ka lipine plastik keʻokeʻo ma ka hakahaka o nā poʻi.
He paʻakikī ka paʻi waho.A he mea paʻakikī ke ʻoki a hōʻino.Hiki iā ia ke pale pono i kāu mau huahana.E hele mākou i ka lipine plastik i waho e hoʻoponopono i kēlā me kēia ʻāpana.A inā pono, e hoʻopaʻa ʻia mākou me nā kiʻi ʻoniʻoni ma waho.
ʻO Fmoc-D-Tyr(tBu)-OH, i ʻike ʻia hoʻi ʻo Fmoc-O-tert-butyl-D-tyrosine, he ʻakika amino i hoʻololi ʻia me nā waiwai kemika kū hoʻokahi.Loaʻa iā ia nā noi i ka biochemistry, ka ʻike lāʻau, ka peptide synthesis, a me ka synthesis organik.
Ka noiʻi biochemical:
Hiki ke hoʻohana ʻia ʻo Fmoc-D-Tyr(tBu)-OH e aʻo i ke ʻano o ka protein a me ka hana.Ma muli o kona ʻano kemika kikoʻī, hiki iā ia ke launa pū me nā molekala ʻē aʻe, e hāʻawi ana i nā ʻike i ka pilina protein-protein, nā pilina protein-ligand, a me nā mea hou aku.
ʻIke lāʻau lapaʻau:
I ka loaʻa ʻana o ka lāʻau lapaʻau, lawelawe ʻo Fmoc-D-Tyr(tBu)-OH ma ke ʻano he koʻikoʻi koʻikoʻi no ka synthesis o nā peptides hoʻoikaika biologically a me nā lāʻau lapaʻau.Ma o nā hoʻololi kemika, hiki ke hoʻomākaukau ʻia nā pūhui me ka waiwai lāʻau lapaʻau no nā haʻawina lāʻau lapaʻau a me pharmacokinetic hou aʻe.
ʻO ka peptide Synthesis:
He kuleana koʻikoʻi ʻo Fmoc-D-Tyr(tBu)-OH i ka synthesis peptide solid-phase.Mahalo i kāna pūʻulu pale Fmoc, ʻae ia i ka pilina amino acid maikaʻi a me ka deprotection, e hiki ai i ka synthesis o nā kaulahao peptide me nā ʻano kikoʻī a me nā hana.
ʻO ka hoʻohuihui kino:
I loko o ka hoʻoheheʻe ʻia, Fmoc-D-Tyr(tBu)-OH e lawelawe ana ma ke ʻano he hale kūkulu hale no ke kūkulu ʻana i nā molekala organik paʻakikī.Ke hoʻohana nei i kāna ʻano kemika kikoʻī, hiki iā ia ke hana i nā hopena kemika me nā molekole ʻē aʻe, e alakaʻi ana i ka hoʻokumu ʻana o nā molekala organik me nā hana i makemake ʻia.
L-Tyr-OH | D-Tyr-OH | DL-Tyr |
N-Me-Tyr(me)-OH | N-Me-Tyr(me).HCL | L-Tyr(tbu)-OH |
L-Tyr-Otbu | D-Tyr-Otbu | Boc-N-Me-L-Tyr(tbu)-OH |
Boc-Tyr-Otbu | Boc-D-Tyr-Otbu | Boc-DL-Tyr-Otbu |
Boc-L-Tyr(tBu)-OH | Boc-Tyr(Akā).Aib | Fmoc-N-Me-Tyr(tBu)-OH |
Fmoc-N-Me-D-Tyr(tBu)-OH | Fmoc-L-Tyr(tBu)-OH | Fmoc-D-Tyr(Et)-OH |
Cbz-N-Me-Tyr(tbu)-OH | Z-Tyr(tbu) | Z-Tyr(tbu).DCHA |
1. Hāʻawi mākou i ka hana maʻamau i nā huahana āu e pono ai.
2. Hiki ke hāʻawi ʻia ke kumukūʻai kiʻekiʻe & hoʻokūkū.
3.We hoʻohiki i kahi papa piha o ka lawelawe ukali no ʻoe.
4. Hiki ke hā'awi 'ia ka nīnau nīnau mea kū'ai a me nā palapala 'ike loea inā noi ma hope o ka hālāwai 'ana i kekahi huina.
5. Nui ma hope o ke kūʻai aku ʻana a i ʻole ka hōʻoia ʻana: E hoʻoponopono koke ʻia kāu nīnau i ka hiki.
6. E hoʻokuʻu aku i nā huahana hoʻokūkū a hoʻokuʻu aku iā lākou i waho i nā mea nui i kēlā me kēia makahiki.
7.Free hāpana hāʻawi ʻia e nānā i ka maikaʻi ma mua o kekahi uku.