NUI
No nā nīnau e pili ana i kā mākou huahana a i ʻole ka papa inoa kumukūʻai, e ʻoluʻolu e waiho i kāu leka uila iā mākou a e hoʻopili mākou i loko o 24 mau hola.
NINAU ANOKūlana halihali
Hiki i ka wela maʻamau
Manaʻo i ke ʻano hoʻouna
Ma ka lewa Ma ka aina Ma ke kai
Kūlana mālama:
E mālama i kahi pōʻeleʻele, Inert ea, lumi wela
ola mālama
Ma kahi o 2 makahiki
Ka nui o ke kauoha liʻiliʻi:
1kg (kūkākūkā)
palapala hōʻoia:COA, wahi hehee, Hoʻololi kikoʻī [α]D20
E hoʻouna mākou i nā ʻikepili āu e makemake ai.Hiki iā ʻoe ke ʻike i ka ʻikepili ma mua ma ke ʻano he kuhikuhi.
No nā huahana maʻamau, hoʻouna mākou i ka ʻikepili hou ma hope o ka puka ʻana mai.
Boc-N-Methyl-DL-alanine;
Alanine, N-[(1,1-dimethylethoxy)carbonyl]-N-methyl-
Hoʻohana pinepine ʻia lākou no ka hoʻopili ʻana i ka pauka.Pili ia i nā koi o nā mea kūʻai aku.No laila, e haʻi mai iā mākou i kāu koi ma mua o ka hoʻopaʻa ʻana inā loaʻa iā ʻoe nā noi kūikawā.
1kg a 25kg He paʻakikī ka paʻi waho.A he mea paʻakikī ke ʻoki a hōʻino.Hiki iā ia ke pale pono i kāu mau huahana.
ʻO ka peptide Synthesis:
He kuleana koʻikoʻi ʻo Boc-N-Me-DL-Ala-OH i ka hana peptide.Me ka hui pale ʻo Boc (tert-butyloxycarbonyl) ma kāna N-terminus, hiki iā ia ke lawelawe ma ke ʻano he amino acid hoʻomaka a i ʻole he ʻāpana waena no ka hoʻolōʻihi ʻana i nā kaulahao peptide.
Ma o ka solid-phase peptide synthesis a i ʻole nā hana synthetic ʻē aʻe, hiki ke hoʻopili ʻia ʻo Boc-N-Me-DL-Ala-OH me nā amino acids a i ʻole nā ʻāpana peptide e kūkulu i nā peptides a i ʻole nā ʻāpana protein me nā kaʻina kikoʻī a me nā hana.
Ke noiʻi ʻana i ka biochemical a me ka molecular biology:
Hiki ke hoʻohana ʻia ʻo Boc-N-Me-DL-Ala-OH ma ke ʻano he probe a lepili paha i nā haʻawina biochemical a me ka molecular biology.Ma ka hoʻokomo ʻana iā ia i loko o nā ʻōnaehana olaola, hiki i nā mea noiʻi ke noiʻi i kāna mau pilina me nā protein, enzymes, a i ʻole biomolecules ʻē aʻe, a laila e hōʻike ana i nā hana hoʻoponopono e pili ana i nā kaʻina olaola.
Eia kekahi, ma muli o kāna hoʻololi ʻana i ka N-methylation, hiki i ka Boc-N-Me-DL-Ala-OH ke loaʻa i nā hana kūlohelohe kūʻokoʻa a i ʻole ka paʻa, e hiki ai ke ʻimi i nā hana olaola hou a i ʻole ka hoʻomohala ʻana i nā biomarkers hou.
Hoʻolālā a me ka hoʻomohala ʻana i ka lāʻau lapaʻau:
Loaʻa iā Boc-N-Me-DL-Ala-OH ka mea hiki i ka hoʻolālā lāʻau a me ka hoʻomohala ʻana.ʻO kona ʻano kemika kū hoʻokahi a me kāna hana olaola hiki ke hoʻolilo iā ia i lāʻau moho a i ʻole ka lāʻau mua.
Ma o ka hoʻolālā a me ka hoʻololi ʻana, hiki i nā mea noiʻi ke ʻimi i ka hiki o Boc-N-Me-DL-Ala-OH i ka mālama ʻana i nā maʻi a i ʻole nā kūlana kūikawā, e hāʻawi ana i kahi kumu kumu no kona noʻonoʻo ʻana ma ke ʻano he moho lāʻau.
Mea ʻepekema a me nā noi:
Hiki ke lawelawe ʻo Boc-N-Me-DL-Ala-OH ma ke ʻano he monomer hana a i ʻole hoʻohui i ka synthesis waiwai.Ma o ka hopena a i ʻole ka polymerization me nā mea kemika ʻē aʻe, hiki ke hoʻomākaukau ʻia nā polymers a i ʻole nā kiʻi ʻoniʻoni me nā waiwai kikoʻī.
Loaʻa kēia mau mea i nā noi ma nā wahi e like me ka biomedical engineering, tissue engineering, a me nā ʻōnaehana lawe lāʻau, e hāʻawi ana i kahi kumu waiwai hou no ka holomua ma nā ʻano pili.
L-ALa | DL-ALA | D-ALa | HN-Me-L-Ala-OH |
N-Me-L-Ala-OMe | N-Me-L-Ala-Ome.Hcl | Boc-N-Me-Ala-OH | HN-Me-L-Ala-OH |
Fmoc-LN-Me-Ala-OH | Fmoc-N-Me-D-Ala-OH | Cbz-N-Me-D-Ala-OH | Cbz-N-Me-L-Ala-OH |
1. No ka MOQ: Hiki iā mākou ke hoʻololi e like me kāu koi.
2. E pili ana i ka hāpana: Pono ka hāpana i ka uku hōʻailona, hiki ke lawe i ka ukana e hōʻiliʻili aiʻole hiki iāʻoe ke uku iā mākou ma mua.
3. Mold workshop, hiki ke hoʻopilikino ʻia e like me ka helu o nā hiʻohiʻona.
4. Hāʻawi mākou i ka lawelawe maikaʻi loa.Ke hana nei kekahi hui kūʻai akamai iā ʻoe.
5. E pili ana i ka hoʻololi: E ʻoluʻolu e leka uila a kamaʻilio mai iaʻu ma kou ʻoluʻolu.