NUI
No nā nīnau e pili ana i kā mākou huahana a i ʻole ka papa inoa kumukūʻai, e ʻoluʻolu e waiho i kāu leka uila iā mākou a e hoʻopili mākou i loko o 24 mau hola.
NINAU ANOKūlana halihali
Hiki ke lawe ʻia ma ka wela maʻamau.
Manaʻo i ke ʻano hoʻouna
ma ka lewa, ma ka aina a ma ke kai paha
Kūlana mālama:
2-8 ° C, pono e mālama ʻia i loko o kahi kūlana i hoʻopaʻa ʻia, anu a me ka lā ʻole.
ola mālama
ma kahi o 2 makahiki
Ka nui o ke kauoha liʻiliʻi:
1kg (kūkākūkā)
palapala hōʻoia:COA, wahi hehee, Hoʻololi kikoʻī [α]D20 E hoʻouna mākou i nā ʻikepili āu e makemake ai.Akā no nā huahana maʻamau, hoʻouna mākou i ka ʻikepili hou ma hope o ka puka ʻana mai.
ʻOiaʻiʻo, hiki iā ʻoe ke ʻike i ka ʻikepili ma mua ma ke ʻano he kuhikuhi.
FMoc-Lys(Mtt)-OH
N-α-FMoc-N-δ-Methyltrityl-L-lysine;
(S)-2-((((9H-fluoren-9-yl)Methoxy)carbonyl)(trityl)aMino)-6-(MethylaMino)hexanoicacid;
N^a-FMoc-N^e-(4-Methyltrityl)-L-lysine
Nalpha-[(9H-Fluoren-9-ylmethoxy)carbonyl]-Nepsilon-(4-methyltrityl)-L-lysine;
Nα-[(9H-Fluoren-9-ylmethoxy)carbonyl]-Nε-(4-methyltrityl)-L-lysine;
Nα-Fmoc-Nε-methyltrityl-L-lysine
(9H-Fluoren-9-yl)MethOxy]CarbonylL-Lys(Mtt)-OH
Hoʻohana pinepine ʻia lākou no ka hoʻopili ʻana i ka pauka.Pili ia i nā koi o nā mea kūʻai aku.No laila, e haʻi mai iā mākou i kāu koi ma mua o ka hoʻopaʻa ʻana inā loaʻa iā ʻoe nā noi kūikawā.
1kg a 25kg He paʻakikī ka paʻi waho.A he mea paʻakikī ke ʻoki a hōʻino.Hiki iā ia ke pale pono i kāu mau huahana.
Solid-Phase Peptide Synthesis (SPPS): Ma SPPS, hoʻohui ʻia nā peptides ma kahi kākoʻo paʻa, maʻamau he resin.Hoʻohana ʻia ʻo 167393-62-6 Fmoc-L-Lys(Mtt)-OH e like me kekahi o nā poloka kūkulu no ka peptide synthesis.ʻO ka hui Fmoc (9-fluorenylmethoxycarbonyl) he pūʻulu pale i hoʻohana mau ʻia ma SPPS no ka pale ʻana i ka hui amino o nā waikawa amino, e pale ana i nā hopena ʻaoʻao makemake ʻole i ka wā o ka hoʻokumu ʻana i ka peptide bond.ʻO ka hui Mtt (4-methyltrityl) kekahi hui pale, hoʻohana pinepine ʻia e pale i ka hui epsilon amino o nā koena lysine.Hāʻawi kēia i ka deprotection koho a me ka hoʻopili ʻana i nā hopena i ka wā elongation peptide.
Hoʻopau koho: 167393-62-6 Fmoc-L-Lys(Mtt)-OH hiki ke koho ʻia ma lalo o nā kūlana haʻahaʻa, maʻamau me ka hoʻohana ʻana i kahi waikawa e like me ka trifluoroacetic acid (TFA).Hōʻike kēia i ka pūʻulu amino o lysine, e ʻae iā ia e komo i ka hoʻokumu ʻana i ka pilina peptide me ka waikawa amino e hiki mai ana i ke kaʻina.
Hoʻohana pinepine ʻia ʻo Fmoc-L-Lys(Mtt)-OH me nā ʻakika amino pale ʻē aʻe Fmoc me nā pūʻulu pale like ʻole e ʻae i ka synthesis o nā peptides paʻakikī me nā pūʻulu hana i pale ʻia.Hoʻopili kēia hoʻolālā i ka pale ʻana a me ka hoʻopili ʻana i nā waikawa amino kikoʻī i kahi kaʻina i koho mua ʻia e kūkulu i ke kaulahao peptide i makemake ʻia.
Hoʻololi Peptide: 167393-62-6 Fmoc-L-Lys(Mtt)-OH hiki ke lawelawe ma ke ʻano he lima no nā hoʻololi hou.Ma hope o ka peptide synthesis, hiki ke hoʻoneʻe ʻia ka hui Mtt, e waiho ana i kahi hui amino manuahi ma ke koena lysine no ka hoʻohui ʻana me nā molekala ʻē aʻe a i ʻole no ka hana hou aku.
Fmoc-His(Mmt)-OH | Fmoc-D-His(Mmt)-OH |
1. No ka MOQ: Hiki iā mākou ke hoʻololi e like me kāu koi.
2. E pili ana i ka hāpana: Pono ka hāpana i ka uku hōʻailona, hiki ke lawe i ka ukana e hōʻiliʻili aiʻole hiki iāʻoe ke uku iā mākou ma mua.
3. Mold workshop, hiki ke hoʻopilikino ʻia e like me ka helu o nā hiʻohiʻona.
4. Hāʻawi mākou i ka lawelawe maikaʻi loa.Ke hana nei kekahi hui kūʻai akamai iā ʻoe.
5. E pili ana i ka hoʻololi: E ʻoluʻolu e leka uila a kamaʻilio mai iaʻu ma kou ʻoluʻolu.