NUI
No nā nīnau e pili ana i kā mākou huahana a i ʻole ka papa inoa kumukūʻai, e ʻoluʻolu e waiho i kāu leka uila iā mākou a e hoʻopili mākou i loko o 24 mau hola.
NINAU ANOKūlana halihali
Hiki i ka wela maʻamau
Manaʻo i ke ʻano hoʻouna
Ma ka lewa Ma ka aina Ma ke kai
Kūlana mālama:
E mālama i kahi pōʻeleʻele, Inert ea, lumi wela
Ola ola
Ma kahi o 2 makahiki
Ka nui o ke kauoha liʻiliʻi:
1kg (kūkākūkā)
palapala hōʻoia:COA, wahi hehee, Hoʻololi kikoʻī [α]D20
E hoʻouna mākou i nā ʻikepili āu e makemake ai.Hiki iā ʻoe ke ʻike i ka ʻikepili ma mua ma ke ʻano he kuhikuhi.
No nā huahana maʻamau, hoʻouna mākou i ka ʻikepili hou ma hope o ka puka ʻana mai.
N-ALPHA-CARBOBENZOXY-N-ALPHA-METHYL-L-PHENYLALANINE;
N-CBZ-N-METHYL-L-PHENYLALANINE;
ZN-METHYL-L-PHENYLALANINE;
Z-MEPHE-OH;
ZN-ME-PHENYLALANINE;
ZN-ME-PHE-OH;
CBZ-N-ME-PHE-OH;
CBZ-N-METHYLPHENYLALANINE
Hoʻohana pinepine ʻia lākou no ka hoʻopili ʻana i ka pauka.Pili ia i nā koi o nā mea kūʻai aku.No laila, e haʻi mai iā mākou i kāu koi ma mua o ka hoʻopaʻa ʻana inā loaʻa iā ʻoe nā noi kūikawā.
1kg a 25kg He paʻakikī ka paʻi waho.A he mea paʻakikī ke ʻoki a hōʻino.Hiki iā ia ke pale pono i kāu mau huahana.
ʻO ka Peptide Synthesis: ZN-Me-L-Phe-OH ke kuleana koʻikoʻi i ka hana peptide synthesis paʻa.ʻO ka pale benzyloxycarbonyl (Z) ma ka N-terminus a me ka pale N-methyl ma ke kaulahao ʻaoʻao e hāʻawi i ka koho a me ka paʻa i ka wā o ka hana peptide elongation.Hāʻawi kēia i ka hoʻopili ʻana i ka stepwise a me ka hoʻonohonoho pono ʻana o nā waikawa amino ma ke kākoʻo paʻa, e hiki ai ke hoʻohui i nā peptides me nā ʻano kikoʻī a me nā hale.
ʻIke Laʻau Laʻau: ZN-Me-L-Phe-OH hiki ke lawelawe ma ke ʻano he ʻāpana i nā hana ʻimi lāʻau.Ma muli o kāna ʻano kemika kūʻokoʻa, hiki ke hoʻohui ʻia i nā moho lāʻau e hoʻomaikaʻi i kā lākou hana, kūpaʻa, a i ʻole nā mea pharmacokinetic.Hoʻohui ʻia, hiki ke hoʻohana ʻia e noiʻi i nā ʻano hana pili ma waena o nā lāʻau lapaʻau a me nā molekala olaola.
ʻEnekinia Protein: Ma ka ʻenekinia protein, hiki ke hoʻohana ʻia ʻo ZN-Me-L-Phe-OH e hoʻololi a ʻenekinia paha i ke ʻano a me ka hana o nā protein.Ma ka hoʻokomo ʻana i kēia pūhui i nā kūlana kikoʻī i loko o kahi protein, hiki ke hoʻololi i ke kūpaʻa o ka protein, ka hana, ka pilina, a i ʻole nā mea biophysical ʻē aʻe, e alakaʻi ana i ka hana ʻana i nā protein hou me nā hana a i ʻole nā ʻano i makemake ʻia.
Noiʻi Biochemical: Hiki iā ZN-Me-L-Phe-OH ke lawelawe ma ke ʻano he mea hana waiwai i ka noiʻi biochemical.ʻO kāna ʻano kemika kikoʻī a me ka hoʻoulu ʻana e hiki ke hoʻohana ʻia i ke aʻo ʻana i ke ʻano a me ka hana o nā molekala olaola e like me nā enzymes, receptors, a i ʻole nā protein.Ma o ka launa pū ʻana me nā molekala a i ʻole nā pūhui ʻē aʻe, hiki iā ia ke hōʻike i nā ʻano hana o ka launa pū ʻana a me nā ala pane i loko o nā ʻōnaehana olaola.
Boc-N-Me-L-Phe-OH | N-Me-D-Phe | Fmoc-N-Me-L-Phe-OH | BOC-N-Me-D-Phe-OH |
FMOC-N-Me-D-Phe-OH | Boc-N-Me-L-Phe-OH.DCHA | Boc-DN-Me-Phe.DCHA | Fmoc-N-Me-D-Leu-OH |
Boc-N-Me-DL-Phe-OH | HN-Me-L-Phe-OH | HN-Me-L-Phe.Hcl |
1. No ka MOQ: Hiki iā mākou ke hoʻololi e like me kāu koi.
2. E pili ana i ka hāpana: Pono ka hāpana i ka uku hōʻailona, hiki ke lawe i ka ukana e hōʻiliʻili aiʻole hiki iāʻoe ke uku iā mākou ma mua.
3. Mold workshop, hiki ke hoʻopilikino ʻia e like me ka helu o nā hiʻohiʻona.
4. Hāʻawi mākou i ka lawelawe maikaʻi loa.Ke hana nei kekahi hui kūʻai akamai iā ʻoe.
5. E pili ana i ka hoʻololi: E ʻoluʻolu e leka uila a kamaʻilio mai iaʻu ma kou ʻoluʻolu.