NUI
No nā nīnau e pili ana i kā mākou huahana a i ʻole ka papa inoa kumukūʻai, e ʻoluʻolu e waiho i kāu leka uila iā mākou a e hoʻopili mākou i loko o 24 mau hola.
NINAU ANOKūlana halihali
Hiki i ka wela maʻamau
Manaʻo i ke ʻano hoʻouna
Ma ka lewa Ma ka aina Ma ke kai
Kūlana mālama:
E mālama i kahi pōʻeleʻele, Inert ea, lumi wela
ola mālama
Ma kahi o 2 makahiki
Ka nui o ke kauoha liʻiliʻi:
1kg (kūkākūkā)
palapala hōʻoia:COA, wahi hehee, Hoʻololi kikoʻī [α]D20
E hoʻouna mākou i nā ʻikepili āu e makemake ai.Hiki iā ʻoe ke ʻike i ka ʻikepili ma mua ma ke ʻano he kuhikuhi.
No nā huahana maʻamau, hoʻouna mākou i ka ʻikepili hou ma hope o ka puka ʻana mai.
(R)-2-((benzyloxycarbonyl)(methyl)amino)-3-methylbutanoicacid;
Cbz-N-Methyl-D-Valine;
N-Methyl-N-Cbz-D-valine;
N-Methyl-N-[(phenylmethoxy)carbonyl]-D-valine;
N-Benzyloxycarbonyl-N-methyl-D-valine;
Cbz-N-Me-D-Val-OH;
(2R)-2- {[(benzyloxy)carbonyl](methyl)amino}-3-methylbutanoicacid;
N-Cbz-N-methyl-D-valine
Hoʻohana pinepine ʻia lākou no ka hoʻopili ʻana i ka pauka.Pili ia i nā koi o nā mea kūʻai aku.No laila, e haʻi mai iā mākou i kāu koi ma mua o ka hoʻopaʻa ʻana inā loaʻa iā ʻoe nā noi kūikawā.
1kg a 25kg He paʻakikī ka paʻi waho.A he mea paʻakikī ke ʻoki a hōʻino.Hiki iā ia ke pale pono i kāu mau huahana.
Peptide Synthesis: ʻO Cbz-N-Me-D-Val-OH ka mea hana ma ke ʻano he hale kūkulu koʻikoʻi i ka synthesis peptide solid-phase.ʻO kāna hui pale benzoyl (Cbz) e hōʻoia i ka paʻa i ka wā o ka synthesis, ʻoiai ka N-methylation e hoʻonui i ka solubility a me ka reactivity.Hāʻawi kēia i ka hoʻokomo maikaʻi ʻana o nā koena D-valine i loko o nā kaulahao peptide, ka hopena i nā peptides maʻemaʻe kiʻekiʻe me nā hana biological makemake.
Haʻawina Koolaola: Pāʻani koʻikoʻi nā peptide i nā kaʻina hana olaola like ʻole, e like me ka hoʻololi ʻana i ka hōʻailona, ka hoʻoponopono ʻana i ka enzyme, a me ka pane kūlohelohe.ʻO ka loaʻa ʻana o Cbz-N-Me-D-Val-OH e hiki ai i nā mea noiʻi ke synthesize i nā peptides i loaʻa nā koena D-valine, e hiki ai iā lākou ke noiʻi i nā kuleana kikoʻī a me nā pilina o kēia mau peptides i nā ʻōnaehana olaola.
ʻIke a me ka hoʻomohala ʻana i ka lāʻau lapaʻau: Ua loaʻa i nā lāʻau lapaʻau-peptide ka nānā nui ʻana i nā makahiki i hala iho nei ma muli o ko lākou kikoʻī kiʻekiʻe a me ka haʻahaʻa haʻahaʻa.ʻO Cbz-N-Me-D-Val-OH, ma ke ʻano he mea nui i ka peptide synthesis, hiki ke hoʻohana ʻia i ka hoʻolālā a me ka hoʻomohala ʻana i nā lāʻau peptide hou.Ma ka hoʻokomo ʻana i nā koena D-valine i loko o kēia mau lāʻau lapaʻau, hiki i nā mea noiʻi ke hana i nā lāʻau lapaʻau ʻoi aku ka maikaʻi me nā waiwai kūlohelohe kūʻokoʻa.
L-Val-OH | DL-Val | HD-Val-OH | N-Me-L-Val-OH |
N-Me-L-Val.HCl | N-Me-D-Val | N-Me-D-Val-OH·HCl | N-Me-L-Val-Ome.HCl |
N-Me-D-Val-Ome.HCl | N-Me-Val-Obzl.HCl | N-Me-D-Val-Obzl.HCl | N-Me-Val-Obzl.Tos |
Boc-N-Me-Val-OH | Boc-N-Me-D-Val-OH | Fmoc-N-Me-Val-OH | Fmoc-N-Me-DL-Val-OH |
Fmoc-N-Me-D-Val-OH | Cbz-N-Me-L-Val-OH |
1. No ka MOQ: Hiki iā mākou ke hoʻololi e like me kāu koi.
2. E pili ana i ka hāpana: Pono ka hāpana i ka uku hōʻailona, hiki ke lawe i ka ukana e hōʻiliʻili aiʻole hiki iāʻoe ke uku iā mākou ma mua.
3. Mold workshop, hiki ke hoʻopilikino ʻia e like me ka helu o nā hiʻohiʻona.
4. Hāʻawi mākou i ka lawelawe maikaʻi loa.Ke hana nei kekahi hui kūʻai akamai iā ʻoe.
5. E pili ana i ka hoʻololi: E ʻoluʻolu e leka uila a kamaʻilio mai iaʻu ma kou ʻoluʻolu.