NUI
No nā nīnau e pili ana i kā mākou huahana a i ʻole ka papa inoa kumukūʻai, e ʻoluʻolu e waiho i kāu leka uila iā mākou a e hoʻopili mākou i loko o 24 mau hola.
NINAU ANOKūlana halihali
Hiki ke lawe ʻia ma ka wela maʻamau.
Manaʻo i ke ʻano hoʻouna
ma ka lewa, ma ka aina a ma ke kai paha
Kūlana mālama:
2-8 ° C, pono e mālama ʻia i loko o kahi kūlana i hoʻopaʻa ʻia, anu a me ka lā ʻole.
ola mālama
ma kahi o 2 makahiki
Ka nui o ke kauoha liʻiliʻi:
1kg (kūkākūkā)
palapala hōʻoia:COA, wahi hehee, Hoʻololi kikoʻī [α]D20 E hoʻouna mākou i nā ʻikepili āu e makemake ai.Akā no nā huahana maʻamau, hoʻouna mākou i ka ʻikepili hou ma hope o ka puka ʻana mai.
ʻOiaʻiʻo, hiki iā ʻoe ke ʻike i ka ʻikepili ma mua ma ke ʻano he kuhikuhi.
Hoʻohana pinepine ʻia lākou no ka hoʻopili ʻana i ka pauka.Pili ia i nā koi o nā mea kūʻai aku.No laila, e haʻi mai iā mākou i kāu koi ma mua o ka hoʻopaʻa ʻana inā loaʻa iā ʻoe nā noi kūikawā.
1kg a 25kg He paʻakikī ka paʻi waho.A he mea paʻakikī ke ʻoki a hōʻino.Hiki iā ia ke pale pono i kāu mau huahana.
Ka noiʻi biochemical:
I loko o ka biochemistry, Cbz-N-Me-Tyr(tbu)-OH ka mea maʻamau i ka synthesis o nā kaulahao peptide a me nā protein ma ke ʻano he hui pale manawaleʻa i ka wā o ka hana synthetic.Mālama kāna mau pūʻulu tert-butyl a me carbobenzoxy i ka hydroxyl a me nā pūʻulu amino o tyrosine, e pale ana i nā hopena kemika makemake ʻole i ka wā synthesis.
ʻIke lāʻau lapaʻau:
I ka loaʻa ʻana o ka lāʻau lapaʻau, lawelawe ʻo Cbz-N-Me-Tyr(tbu)-OH ma ke ʻano he koʻikoʻi koʻikoʻi no ka synthesis o nā peptides hana biologically a me nā lāʻau lapaʻau.Ma ka hoʻokomo ʻana i kēia derivative, hiki i nā mea noiʻi ke synthesize i nā kaulahao peptide me nā ʻano kikoʻī a me nā hoʻolālā, ka nānā ʻana i nā pūhui me ka waiwai pharmacological.
ʻO ka peptide Synthesis:
He kuleana koʻikoʻi ka Cbz-N-Me-Tyr(tbu)-OH i ka peptide synthesis.Hiki ke hoʻohana ʻia ma ke ʻano he mea hoʻomaka a i ʻole synthetic intermediate, e komo ana i ka synthesis o nā kaulahao peptide me nā ʻano kikoʻī a me nā hana.Ma ka hoʻokomo mālie ʻana i nā koena amino acid ʻē aʻe, hiki i nā mea noiʻi ke synthesize i nā molekala peptide me nā hana koʻikoʻi.
ʻO ka hoʻohuihui kino:
I loko o ka hoʻoheheʻe ʻia, Cbz-N-Me-Tyr(tbu)-OH ka mea hana ma ke ʻano he hale kūkulu hale no ke kūkulu ʻana i nā molekala organik paʻakikī.ʻO kāna ʻano kemika kikoʻī e hiki ai iā ia ke hana i nā hopena kemika kikoʻī me nā molekole ʻē aʻe i ka wā o ke kaʻina hana synthetic, e alakaʻi ana i ka hoʻokumu ʻana o nā molekala organik me nā hana i makemake ʻia.
N-Me-Tyr(Me)-OH | Boc-N-Me-L-Tyr(tbu)-OH | Fmoc-N-Me-L-Tyr(tBu)-OH | FMOC-N-Me-D-Tyr(me)-OH |
Fmoc-N-Me-D-Tyr(tBu)-OH | Boc-N-Me-L-Tyr(bzl)-OH | Fmoc-N-Me-L-Tyr(bzl)-OH | Boc-N-Me-D-Tyr(bzl)-OH |
1. No ka MOQ: Hiki iā mākou ke hoʻololi e like me kāu koi.
2. E pili ana i ka hāpana: Pono ka hāpana i ka uku hōʻailona, hiki ke lawe i ka ukana e hōʻiliʻili aiʻole hiki iāʻoe ke uku iā mākou ma mua.
3. Mold workshop, hiki ke hoʻopilikino ʻia e like me ka helu o nā hiʻohiʻona.
4. Hāʻawi mākou i ka lawelawe maikaʻi loa.Ke hana nei kekahi hui kūʻai akamai iā ʻoe.
5. E pili ana i ka hoʻololi: E ʻoluʻolu e leka uila a kamaʻilio mai iaʻu ma kou ʻoluʻolu.