Hana Kiʻekiʻe Liquid Chromatography/ HPLC
ʻIke ʻia ʻo High Performance Liquid Chromatography/HPLC ma ke ʻano he "high-pressure liquid chromatography", "high-speed liquid chromatography", "high-resolution liquid chromatography", "modern column chromatography", etc. High performance liquid chromatography is a important branch o ka chromatography.Hoʻohana ʻo ia i ka wai e like me ka mobile phase a hoʻohana i kahi ʻōnaehana infusion kiʻekiʻe e paʻi i nā solvents hoʻokahi me nā polarities like ʻole a i ʻole nā solvents hui pū ʻia, buffers a me nā ʻāpana kelepona ʻē aʻe me nā ʻano like ʻole i loko o kahi pae paʻa.Ma hope o ka hoʻokaʻawale ʻia ʻana o nā ʻāpana o ke kolamu chromatographic, komo lākou i ka mea ʻike no ka ʻike a me ka nānā ʻana o ka hāpana.Ua lilo kēia ʻano hana i ʻenehana hoʻokaʻawale a me ka nānā ʻana i ka ʻenehana kemika, lāʻau lapaʻau, ʻoihana, agronomy, nānā ʻana i nā mea kūʻai aku a me ka nānā kānāwai.
Nā hiʻohiʻona o ka chromatography wai hana kiʻekiʻe:
① ʻO ke kaomi kiʻekiʻe: He wai ka pae kelepona.Ke kahe ʻo ia ma ke kolamu chromatographic, ʻoi aku ke kūʻē ʻana.I mea e hele wikiwiki ai i ke kolamu chromatographic, pono e kaomi i ka wai lawe.
②High pono: kiʻekiʻe kaʻawale kaʻawale.Hiki ke koho ʻia ka pae paʻa a me ka pae kelepona e hoʻokō i ka hopena hoʻokaʻawale maikaʻi loa, ʻoi aku ka nui o nā manawa ma mua o ka hoʻokaʻawale ʻana o nā hale kiaʻi distillation ʻoihana a me ka chromatography gas.
③High sensitivity: hiki i ka UV detector ke hiki i 0.01ng.
④Wide ākea o nā noi: ʻoi aku ma mua o 70% o nā pūhui organik hiki ke nānā ʻia e ka chromatography wai hana kiʻekiʻe.
⑤ ʻOi aku ka wikiwiki ma mua o ka chromatography wai maʻamau
Eia hou, hiki ke hoʻohana pinepine ʻia ka chromatography wai kiʻekiʻe a me nā kolamu chromatography, ʻaʻole i hōʻino ʻia nā laʻana, a maʻalahi hoʻi e hoʻihoʻi.Eia nō naʻe, loaʻa iā lākou nā hemahema.Ke hoʻohālikelike ʻia me ka chromatography gas, loaʻa iā lākou nā pono ponoʻī a hoʻokō kekahi i kekahi.
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Ka manawa hoʻouna: Nov-23-2023
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